Target: Nucleoside Diphosphate Kinase NCBI RefSeq#: NZ_CP009257.1 *Protein ID (NP or XP #) or Wolbachia#:4W98 Organism (including strain): Acinetobacter baumannii (strain SDF) Etiologic Risk Group (see link below): Group 2 http://www.bacterio.net/-hazard.html#group2
Disease Information: Acinetobacter baumannii typically causes pneumonia and meningitis and outbreaks primarily occur in the ICU. Acinetobacter is a group of bacteria that is found in soil and water, acinetobacter baumannii poses a large threat to those with weakened immune systems. https://www.cdc.gov/hai/organisms/acinetobacter.html
Essentiality of this protein: NDK is essential for bacterial growth, it generates NTPs and helps to spread the bacterial infection to other parts of the body.
Is it a monomer or multimer as biological unit?: multimer Complex of proteins?: no Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b01805
Image of protein (PyMol with features delineated and shown separately):
TargetDiscoProteinImage.PNG
Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
EFMAIERTLSIVKPDAVSKNHIGEIFARFEKAGLKIVATKMKHLSQADAEGFYAEHKERGFFGDLVAFMTSGPVVVSVLE
NCBI RefSeq#: NZ_CP009257.1
*Protein ID (NP or XP #) or Wolbachia#:4W98
Organism (including strain): Acinetobacter baumannii (strain SDF)
Etiologic Risk Group (see link below): Group 2
http://www.bacterio.net/-hazard.html#group2
Disease Information: Acinetobacter baumannii typically causes pneumonia and meningitis and outbreaks primarily occur in the ICU. Acinetobacter is a group of bacteria that is found in soil and water, acinetobacter baumannii poses a large threat to those with weakened immune systems.
https://www.cdc.gov/hai/organisms/acinetobacter.html
Link to TDR Targets page: n/a
Link to Gene Database page:
https://www.ncbi.nlm.nih.gov/genome/?term=Acinetobacter%20baumannii[Organism]&cmd=DetailsSearch
Essentiality of this protein: NDK is essential for bacterial growth, it generates NTPs and helps to spread the bacterial infection to other parts of the body.
https://onlinelibrary.wiley.com/doi/full/10.1046/j.1365-2958.1998.00846.x
Is it a monomer or multimer as biological unit?: multimer
Complex of proteins?: no
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
https://pubs.acs.org/doi/10.1021/acs.jmedchem.6b01805
EC#: 2.7.4.6
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/enzyme.php?ecno=2.7.4.6
-- Show screenshot of BRENDA enzyme mechanism schematic
-- -or link (or citation) to paper that contains assay information
https://www.sciencedirect.com/science/article/abs/pii/S0753332217371895
Structure (PDB or Homology model): 4W98
Current Inhibitors:
http://www.brenda-enzymes.org/enzyme.php?ecno=2.7.4.6&Suchword=inhibitors&reference=&UniProtAcc=&organism%5B%5D=
Expression Information (has it been expressed in bacterial cells): Yes
https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.2910500406
Purification Method: hyperproliferation of neoplastic cells
Image of protein (PyMol with features delineated and shown separately):
EFMAIERTLSIVKPDAVSKNHIGEIFARFEKAGLKIVATKMKHLSQADAEGFYAEHKERGFFGDLVAFMTSGPVVVSVLE
GENAVLAHREILGATNPKEAAPGTIRADFAVSIDENAAHGSDSVASAEREIAYFFADNEICPRTR